polyhistidine
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Polyhistidine-tag — A polyhistidine tag is an amino acid motif in proteins that consists of at least six histidine ( His ) residues, often at the N or C terminus of the protein. It is also known as hexa histidine tag, 6xHis tag, and by the trademarked name His tag… … Wikipedia
Protein purification — is a series of processes intended to isolate a single type of protein from a complex mixture. Protein purification is vital for the characterisation of the function, structure and interactions of the protein of interest. The starting material is… … Wikipedia
ZIC2 — Zic family member 2 (odd paired homolog, Drosophila), also known as ZIC2, is a human gene.cite web | title = Entrez Gene: ZIC2 Zic family member 2 (odd paired homolog, Drosophila)| url = http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene… … Wikipedia
Dihydrofolate reductase — Ribbon diagram of human dihydrofolate reductase in complex with folate (blue). From PDB 1DRF … Wikipedia
Affinity chromatography — is a method of separating biochemical mixtures and based on a highly specific interaction such as that between antigen and antibody, enzyme and substrate, or receptor and ligand. Contents 1 Uses 2 Principle 3 Batch and column setup … Wikipedia
FLAG-tag — FLAG tag, or FLAG octapeptide, is a polypeptide protein tag that is added to a recombinant expressed protein. It is made by inserting a vector into the DNA sequence coding for that specific protein. It can be used for affinity chromatography,… … Wikipedia
Nickel(II) sulfate — Anhydrous Hexahydrate … Wikipedia
CORO1A — Coronin, actin binding protein, 1A, also known as CORO1A, is a human gene.cite web | title = Entrez Gene: CORO1A coronin, actin binding protein, 1A| url = http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene Cmd=ShowDetailView TermToSearch=11151|… … Wikipedia
Cell-free protein array — technology produces protein microarrays by performing in vitro synthesis of the target proteins from their DNA templates. This method of synthesizing protein microarrays overcomes the many obstacles and challenges faced by traditional methods of… … Wikipedia
his tag — (= histidine tag) Epitope tag based on a short stretch (ca 6) of histidine residues. In addition to detection with anti polyhistidine antibodies, such tags permit easy protein purification on nickel based affinity columns … Dictionary of molecular biology
